- Definition of your unique operational requirements for sample management and traceability
- Creation of a complete collection solution to reflect your requirements and your brand
- All-in-one devices for optimal self-collection
- Maximize donor compliance
- Standardize sample collection
- Stabilize nucleic acids at ambient temperature
- Compatible with high-throughput processing
- Suitable for sequencing
- Interact with our informatics experts to gain deep insights into your results
- Intuitive data visualizations for use in publications
- Clear reporting to enable you to focus on results, not trying to interpret the data
- CLIA - Clinical Laboratory Improvement Amendments
- CAP - College of American Pathologists
- State certified where applicable
- Whole blood
- Buffy coat
- Buccal swabs
- And more
- Quantitation – Fluorescent quantitation is commonly used to accurately determine the amount of double stranded DNA in a sample. We use a method involving the fluorescent dye PicoGreen to quantitate all samples that we extract.
- Purity assessment - An A260/A280 ratio is commonly used to assess the purity of a DNA sample following extraction. This can be accomplished by determining the ratio of spectrophotometric absorbance of the sample at 260 nm to that of 280 nm. We perform this assessment using a NanoDrop instrument.
- Integrity assessment - DNA integrity is critical for some downstream applications and to assess this agarose gel electrophoresis is performed to visualize the DNA before it can move on for further analysis. A single band with high molecular weight (>23kb) is an indicator of good sample integrity and a smear or otherwise lower molecular weight sample is an indicator of poor sample integrity.
- Whole Genome Amplification (WGA) – Allows you to amplify the DNA in a precious sample so that it can be used for many assays in the future without the requirement of re-collection.
- Aliquotting – Sample handling that allows you to have the sample distributed across multiple tubes and reduce or eliminate future freeze-thaw damage.
- Storage – Sample storage can be arranged for months or years depending on requirements in a CLIA accredited, environment controlled facility.
- less than 1000 samples
- fewer than 50 SNPs
- Technology we use is TaqMan Single Tube assays. The TaqMan assay is an allele discrimination assay using PCR amplification and a pair of fluorescent dye detectors that target the SNP. One fluorescent dye is attached to the detector that is a perfect match to the first allele (e.g. an “A” nucleotide) and a different fluorescent dye is attached to the detector that is a perfect match to the second allele (e.g. a “C” nucleotide). During PCR, the polymerase will release the fluorescent probe into solution where it is detected using endpoint analysis in a real-time instrument.
- more than 1000 samples
- 50-500 SNPs
- Technology we use is TaqMan OpenArray. This system involves the custom printing of the SNPs of interest onto an array and subsequent automated processing of those arrays.
- more than 100 samples (in some cases can be utilized with smaller sample numbers)
- thousands to millions of SNPs
- VNTRs – Variable number tandem repeats
- CNVs – Copy number variants
- INDELs – Insertions/deletions
- Performed using the Ion Proton and Ion PGM platforms
- Best used for targeted sequencing of large fragments of DNA (e.g. whole chromosome) and whole exome sequencing.
- Best used for targeted sequencing of small to midsized DNA fragments (i.e. 100-1,000bp).
- Fluorescent quantification of dsDNA using PicoGreen
- 260/280 Ratios by UV absorbance (to gauge purity)
- Agarose gels (to gauge DNA integrity)
- Make sure all caps are secure for transport.
- Each sample must have a unique identifier written on the collection tube.
- For information on how to ship buffy coat samples at ambient temperature, please click here to learn about our HEMAgene•BUFFY COAT product.
- For more information on packaging of ambient temperature buffy coat samples, please refer to the following resources:
- Shipping buffy coat samples - PD-WP-00034.pdf
- IATA Guidelines
- Your national biological sample shipping guidelines.
- If you are not using the HEMAgene•BUFFY COAT reagent or another means of ambient temperature stabilization, we recommend that buffy coat samples be shipped via overnight courier on dry ice.
- Overnight ambient or cool temperature (4°C) is recommended for whole blood samples. It is important that the samples not freeze or get too hot to preserve the integrity of the sample.
- For more information on the packaging of whole blood samples please consult with your preferred courier and/or:
- We recommend that extracted DNA samples be shipped via overnight courier on dry ice.
- For more information on packaging of extracted DNA samples please refer to the following resources:
- IATA Guidelines
- Your national biological sample shipping guidelines.
- Identifying Purposes
- Limiting Collection
- Limiting Use, Disclosure and Retention
- Individual Access
- Challenging Compliance
GenoFIND Services for Human Genomics
Turnkey solutions to power genomic research
DNA Genotek offers a complete suite of solutions for every step of your genomic research, from sample to answer. Customize GenoFIND's genomics services by choosing the products and services you need to optimize your study and achieve your goals.
Watch the video to learn more about GenoFIND™
"Your Oragene/saliva kit is really useful. We are using it in many fields of genome research relating to human diseases – for mutation analysis, exome sequencing and GWAS genotyping – resulting in successful publications."
Dr. Shiro Ikegawa
Laboratory of Bone and Joint Diseases, Center for Integrative Medical Sciences, RIKEN, Tokyo, Japan
Professional study design
Expert guidance for your study design.
Engage our experts for correct, statistically valid study designs. You can be confident in our ability to ensure meaningful results.
Online study management tools
Manage participant registration and maximize compliance.
Give your study participants a private, secure interface to register for and engage with your study. Collect relevant, structured metadata and integrate seamlessly with downstream analysis tools.
Study Coordinator Dashboard
Monitor the status of every sample in your study in real-time while tracking compliance data from your participants. Consolidate and view compliance metrics, sample status and more. The Study Coordinator Dashboard integrates with the Participant Portal and Single Order Fulfilment systems to bring together all of the most important study progress metrics.
Customization and logistics
Integrated solutions for cost-effective execution of population-wide projects.
The flexibility of our product customization enables you to focus on your study while DNA Genotek addresses those elements required to fully integrate the sample collection component into your offering. Our services include:
DNA Genotek single order fulfillment services offer our customers the option of leveraging our established shipping and logistics expertise to deliver kits directly to their study participant. When combined with our professional customization services, this offers a full turnkey solution, enabling you to focus on building your business without having to worry about managing supply chain logistics.
Our optimized non-invasive self-collection solutions enable you to scale your project with high quality samples.
DNA Genotek collection products enable easy self-collection of DNA or RNA samples.
Wet lab services
Full service clinical grade laboratory located in the United States with a comprehensive staff of PhD and MD scientists. CLIA and CAP accredited facility and State certified where applicable.
Full-sample DNA extraction (i.e. 4 mL) or aliquot extraction (700 µL) available.
Extractions are performed manually using DNA Genotek’s Et-OH based extraction reagent prepIT®.L2P or automated on the Chemagic magnetic bead extraction platform. The following quality control metrics are performed on each sample: PicoGreen, a fluorometric measurement of DNA concentration, Nanodrop, a spectrophotometric A260/280 absorbance measurement of sample purity, and Agarose Gel, to visualize DNA integrity.
Full-sample (i.e. 4 mL) or aliquot extraction (either 500 µL or 1 mL) available. Extractions are performed manually using Qiagen’s RNeasy kits. The following quality control metrics will be performed on each sample: Nanodrop spectrophotometry for RNA quantification and bioanalyzer electropherogram tracing for visualization of RNA integrity (using Agilent RNA6000 Nano Kit).
Various applications including TaqMan single tube assays, TaqMan OpenArrays, fragment analysis (e.g., genotyping VNTRs, CNVs, indels, microsatellites, etc.) and both Illumina and Affymetrix microarrays.
TaqMan single tube assays offer flexibility in sample batch volume and very high call rates (>98%). Reagents are purchased from ThermoFisher, select from tens of thousands of validated, functionally tested or DME assays. If commercial assays are unavailable we can design custom builds based on peer-reviewed publications.
TaqMan Open Array are economical for custom SNP panels >20 variants and offer the same high call rates as single tube assays. Choose from various formats such as 26, 60, 120, 180 or even 240 SNPs. Panels are manufactured in batches of 960 samples and must be run in 48 sample or 96 sample microtitre plates.
Illumina or Affymetrix microarrays allow simultaneous genotyping of hundreds of thousands of SNPs. We have a fully automated pipeline for microarray processing, experience processing tens of thousands of arrays including several different GWAS BeadChips, the HumanMethylation 450K and Affymetrix 6.0.
Telomere length analysis (TLA)
Is performed using a quantitative real-time PCR (qRT-PCR) method for absolute telomere length determination. A description of the technique used can be found in O'Callaghan, N., V. Dhillon, P. Thomas, and M. Fenech. 2008 BioTechniques 44:807-809.
Quantitative real-time PCR
Select a custom list of mRNA targets for gene expression analysis. Constitutively expressed house-keeping genes are used to create a standard curve to determine up or down regulation of experimental transcripts.
Various Next Generation Sequencing (NGS) platforms offer affordable solutions for both large and small studies while technical expertise ensures reliable data generation.
Illumina - HiSeq4000 offers cost-effective, high-throughput Whole Exome Sequencing (WES), Whole Genome Sequencing (WGS) and RNA-Seq. With the instruments dual flow-cell larger projects can be completed expeditiously. We currently offer research use only WES and clinical WES (both healthy and disease phenotype directed reporting is available). Consent documentation and requisition forms must be filled out prior to ordering clinical WES (typically by a referring doctor). WGS is RUO right now but we are developing a fully validated clinical report.
Illumina - MiSeq is the perfect NGS platform for targeted sequencing projects. Select from various pre-designed commercial panels or design a custom panel for targeted resequencing.
Ion Torrent - Ion Proton is ideal for small sample projects for Whole Exome Sequencing (WES), including clinical WES. The AmpliSeq exome capture library offers 33 Mb coverage of coding exons (97% of consensus coding sequences or cCDS); total design coverage including padding and flanking regions is ~58 Mb. With better sensitivity for single nucleotide variants and the P1V3 chip and Hi-Q chemistry (offers superior read quality for homopolymeric stretches of nucleotides) our lab uses the AmpliSeq library for participation in CAP (College of American Pathologists) proficiency testing for Next-Generation Sequencing.
Sanger sequencing – Confirmatory Sanger sequencing is often used to verify regions or variants of interest of the genome underrepresented during Whole Exome and Whole Genome Sequencing. The customer can provide template DNA or purified PCR product for Sanger Sequencing. With a list of genome targets, the GenoFIND lab can also assist in primer design.
Our experienced bioinformaticians are able to work with raw data that is generated through our wet lab services or that has been generated at another facility. We use a robust, version controlled pipeline and curated taxonomic database to ensure consistent analysis.
Our analytical pipelines combine bioinformatics tools and software engineering best practices like containerization and cloud computing. This lets us deliver fast turnaround-times and larger studies more economically, while maintaining complete version-control of all aspects of the analysis.
Full service clinical grade laboratory located in the United States.
Comprehensive staff of PhD and MD scientists.
Assays & techniques
DNA and RNA Extraction
Accept many sample types including:
Fully validated standard operating procedures (SOPs) are used with a number of automated and manual extraction approaches to obtain the highest quality and yield of DNA regardless of sample type.
Every DNA extraction is accompanied by a number of standard quality controls to ensure accuracy of sample assessment and ultimately better success on downstream applications. Quality control measures include:
Gel electrophoresis image demonstrating DNA samples with varying integrity. A single band showing molecular weight of 23kB or higher such as the first lane (1) show optimal integrity and suggest that the sample should be sufficient for most downstream applications. Smearing and decreasing overall molecular weight such as the samples in the remaining lanes (2-5) suggests lesser sample integrity. Samples with lesser sample integrity may not be sufficient for some downstream applications.
Single Tube SNP Genotyping
Best for projects with:
Best for projects with:
Best for projects with:
Custom Content Available
Fragment Analysis (INDEL, VNTR or CNV)
A technology used to assess insertions or deletions in the gene sequence. We use an approach including polymerase chain reaction (PCR) to amplify the region of interest followed by capillary electrophoresis to determine the length of the fragments amplified.
Fragment analysis allows for the investigation of:
Telomere Length Analysis (TLA)
This service is typically performed using a quantitative real-time PCR (qRT-PCR) method for absolute telomere length that can be found in O'Callaghan, N., V. Dhillon, P. Thomas, and M. Fenech. 2008 BioTechniques 44:807-809.
Other methods may be possible, please contact our Services Coordinator for more information.
We offer two types of sequencing technologies:
Next generation sequencing
What services does GenoFIND offer?
GenoFIND Services offers a wide variety of services including: nucleic acid extraction, genotyping, PCR- based assays, array-based assays and sequencing-based assays. In addition to extraction and downstream applications we also are able to offer a range of scientific consulting, bioinformatics and analysis services. We have access to a wide variety of platforms and qualified scientists with years of experience working with saliva samples so if you have any questions about a specific assay or analysis please contact your Sales Representative or the Services Coordinator for more information.
What should I do if I am not sure exactly what type of assay is the best choice for my project?
Contact your Sales Representative or the Services Coordinator for more information. Based on what you hope to accomplish we can usually recommend the most suitable technology for your project. If some background research is required a consulting scientist can review the literature from your field and make project recommendations.
I am not familiar with genomics. Can you help me to understand the data once my samples have been processed?
Our reports have been designed to be straightforward and easy to read. The Services Coordinator can also help you to understand the report and describe how to interpret it. If more in-depth analysis is required a consulting scientist can review your data and provide further interpretation.
How long will it take for my services to be performed?
The turnaround time for a project will vary dramatically depending on several factors including: number of samples, type of analysis and the queue at the lab. Typically most average sized projects can be completed within 4-5 weeks from the time that samples are received but if you require more rapid turnaround please let the Services Coordinator know. Remember the earlier you are in the queue the easier it will be to accommodate your timelines so always plan ahead.
What quality control measures come standard with your DNA extraction services?
We perform the following QC tests on all samples extracted:
More information under the Assays & techniques tab.
How do I ship my samples?
Oragene or ORAcollect saliva samples – Shipping recommendations
Buffy coat samples – Shipping recommendations
Whole blood – Shipping recommendations
Extracted DNA – Shipping recommendations
What needs to be included on my manifest?
Manifest must list all samples using their UNIQUE identifiers
No extra spaces or punctuations (complete concordance between tube and manifest is required or batch will be put on hold until issues are resolved)
It is critical that all components of the unique identifier are addressed on the manifest including: leading and trailing zeros, initials or other alpha or numeric annotations.
Please click here to view a sample manifest.
Can you store my samples?
If required, short to mid-term storage of samples may be possible for your raw or extracted samples. Please contact the Services Coordinator for more information.
What will happen to my samples once the work is complete?
If you would like unused samples returned to you for storage and future analysis please ensure that you provide return shipping information including a courier account number at the time of contract signing.
Unless otherwise stated all samples that are not returned will be retained for 30 days past the date of project completion before being destroyed.
How long will my data be stored?
Unless otherwise stated, data will be retained for 1 year from the date of project completion on a secure server before being destroyed.
How long will it take for my services to be performed?
If you have any questions about this policy or would like further information on the ten principles please contact: email@example.com.
What is a SNP?
A SNP is a single nucleotide polymorphism and refers to a change in a single base at a specific location in the genome. These point changes in the genetic code may or may not result in changes in the phenotype of the donor. Referred to as a marker if can be used to map inherited variation among individuals, often with reference to traits.
What is an rs#?
An rs# is the reference SNP ID number that is used to identify a specific location within the genome. The rs# is used to find primers and probes that specifically map to that location in the genome and will assist in determining the donors’ genotype at that position.
Dr. Ravinesh Kumar of the Conte Center for Computational Neuropsychiatric Genomics turned to GenoFIND for the analysis of collection samples for his pilot study. See why here.
"Oragene fulfilled all of our requirements for DNA sampling. It was a completely hassle free approach and we generated high quality and pure DNA for association studies and hence successfully accomplished our research project."
Dr. Toolika Singh, Researcher
Infectious Disease Research Laboratory, Banaras Hindu University
Inside DNA Genotek