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Superior Samples • Proven Performance
- Provides a snapshot of the microbial profile at time of collection and prevents microbial growth
- Stabilizes RNA at room temperature and DNA up to 37°C for 3 weeks
- Easy self-collection of high quality DNA and RNA from oral samples
- Eliminates the need for cold chain transportation
- Compatible with downstream applications (e.g., RT-qPCR, microarray)
- Stabilizes samples at point of collection ensuring the DNA and RNA is unchanged at time of processing
- Facilitates simultaneous detection of DNA and RNA from bacteria and viruses all from one sample
- Reduces transportation and storage cost and complexity with 3 weeks of ambient temperature stability of DNA and RNA
- Enables identification of the living or metabolically active bacteria at time of collection through RNA expression profiling
- Format compatible with high-throughput processing increases efficiency and minimizes sample handling errors
OMNIgene•ORAL | OM-505
All-in-one system for the collection and rapid stabilization of microbial nucleic acids.
Microarrays and Next Generation Sequencing give researchers the ability to easily detect and quantitate molecular targets in a highly multiplexed fashion. Due to the complex nature of samples required for metagenomic research of the microbiome, bias can be easily introduced through improper sample collection, stabilization and transport.
Key features
Benefits
Complete donor user instructions for this product are available here.
Supporting evidence
Subject |
Target |
Reference |
Virus |
DNA |
EBV1, HHV-82,3, CMV4 |
|---|---|---|
RNA |
HCV5, HIV5,6 |
|
Bacteria |
DNA |
HOMIM7 |
RNA |
E. coli9 |
|
Extraction systems |
Abbott m20005,6, Qiagen MinElute8 |
|
Downstream applications |
qPCR1,2,3, Microarray7 |
|
1 Stability, recovery efficiency and detection limit of human viral DNA in OMNIgene®•ORAL (OM-505). DNA Genotek. PD-WP-00028.
2 High quality microbial DNA collected with OMNIgene•ORAL enables detection of HHV-8 in saliva. DNA Genotek. MK-00018.
3 Evaluation of a new commercial kit for detection of human herpesvirus 8 (HHV-8) in saliva. http://www.dnagenotek.com/ROW/pdf/GriffithUniversity.pdf
4 Genotyping of cytomegalovirus from toddlers’ saliva samples collected with OMNIgene•ORAL. DNA Genotek. MK-00050.
5 Compatibility of saliva collected using OMNIgene•ORAL (OM-505) with the Abbott m2000rt RealTime System for the detection of viral RNA. DNA Genotek. PD-WP-00025.
6 OMNIgene•ORAL (OM-505) stabilizes RNA from human viruses at room temperature for several weeks. DNA Genotek. PD-WP-00024.
7 OMNIgene•ORAL stabilizes microbial DNA profiles in oral fluid samples, enables more precise characterization of oral flora. DNA Genotek. MK-00090.
8 OM-505 Microbial DNA and RNA purification protocol using QIAGEN® QIAamp® MinElute Virus Spin Kit. DNA Genotek. PD-PR-00214.
9 Internal validation using OM-505 extraction8 in house RT-qPCR.
Literature
OMNIgene•ORAL is for research use only, not for use in diagnostic procedures.
Full terms and conditions for all DNA Genotek products are available here.
DNA Genotek's sample collection devices and nucleic acid stabilization chemistries are protected by issued and pending patents in numerous countries around the world.
By using HOMIM (Human Oral Microbe Identification Microarray), we demonstrated that saliva samples stored in OMNIgene•ORAL were indeed stabilized for at least 3 weeks, even at 37° Celsius. This is outstanding! Consequently, I think OMNIgene•ORAL would be very useful for remote collections alleviating the need for a -80° freezer or, for that matter, any refrigeration to preserve samples. This is an incredible benefit for any investigator who is interested in preserving precious clinical samples, especially for use with molecular analyses of genomic material.
Bruce J. Paster, Ph.D.
Director, Human Microbe Identification Microarray Core
Head, Department of Molecular Genetics, The Forsyth Institute
DNA from saliva vs. blood
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Overcoming challenges in DNA sample collection.
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