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Technical Support: Documents

The following technical documents are available for your reference. Check back often for updates.


Oragene•DNA General Product Information
• Oragene•DNA Genetic Research Product Brochure (pdf)
• Oragene•DNA Clinical Diagnostics Product Brochure (pdf)
• OG-250 Donor Instructions (pdf)
• OG-300 Donor Instructions (pdf)
• OG-100 Donor Instructions (pdf)


Oragene•RNA Documentation

• Oragene•RNA Donor Collection Protocol (pdf)
• Oragene•RNA Purification Protocol for volumes up to 1000 uL (pdf)
• Stability and Yield of RNA collected from saliva using Oragene•RNA for Expression Analysis (pdf)
• Oragene•RNA Product Brochure (pdf)

DNA Collection with Saliva
• DNA Yield with Oragene•DNA (pdf)
• Mailing Solutions (pdf)
• Bacterial DNA Content with Oragene•DNA (pdf)
• Maximizing DNA yield with Oragene•DNA (pdf)
• Increasing DNA yield by collecting more saliva (pdf)
• DNA Yield is Proportional to Saliva Volume (pdf)

DNA Storage and Stability with Oragene•DNA
• Long-term Storage of Oragene•DNA Samples (pdf)
• DNA Stability with Oragene•DNA (pdf)
• GenPlates® for the storage of Oragene•DNA™ samples (pdf)

Manual Purification Protocols
• Laboratory Protocol for Manual Purification of DNA from 0.5 mL of Oragene•DNA / saliva (pdf)
• Laboratory Protocol for Manual Purification of DNA from 4.0 mL of Oragene•DNA / saliva (pdf)
• From turbidity to clarity: Simple methods to improve the A260/A280 ratio of Oragene•DNA purified DNA samples (pdf)
• "Super-Pasteurization" of Oragene•DNA/Saliva samplesl (pdf)
• Oragene•DNA purification using the PUREGENE® DNA Purification Kit (pdf)
• Compatibility of the PUREGENE® DNA Purification Kit with Oragene•DNA DNA
Collection (pdf)
• Comparison of DNA purified with Oragene•DNA and the QIAamp Mini Kit (pdf)

Compatibility with Automated Purification Systems
• Compatibility of the AUTOPURE® with Oragene•DNA DNA Collection (pdf)
• High-throughput DNA purification with the Qiagen BioRobot EZ1 (pdf)
• High-throughput DNA purification with the Magtration® 12GC (pdf)

Downstream Applications and Oragene•DNA
• Detection of LDL receptor mutations by PCR, PCR-RFLP and Southern analysis (pdf)
• Oragene•DNA and DNA sequencing with ABI PRISM® (pdf)
• Oragene•DNA is compatible with TaqMan® SNP genotyping (pdf)
• Oragene•DNA and Whole Genome Amplification with GenomiPhi (pdf)
• Oragene•DNA is ideal for long-range PCR (pdf) 
• SNP genotyping of Oragene•DNA with SNPstream ® (pdf)
• Oragene•DNA™ and GenomePlex® Whole Genome Amplification (pdf)
• Oragene•DNA™ and REPLI-g® Whole Genome Amplification (pdf)
• Sex Determination of Oragene®•DNA samples using ReaX™
Mastermix PCR beads (pdf)

For technical support call 1.613.723.5757 or email us at support@dnagenotek.com

 
The collection protocol is very convenient, the extraction is very quick and simple, and the yield is great. Our downstream applications include PCR, restriction enzyme digestion and sequencing.

We have collected numerous family members who were not willing to go for a blood draw, as well as children, elderly patients and overseas relatives. The ease of shipping and self-collection are remarkable. Our lab is very impressed with this timely product.

Ruth McPherson, MD, PhD
Nihan Kavaslar, PhD
Lipoproteins and Atherosclerosis Research Group
University of Ottawa Heart Institute
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