DNA purification starts
as soon as a donor closes the collection vial and the
saliva is mixed with the Oragene•DNA solution. While the purifier
is included with the Oragene•DNA kits, some researchers and
clinicians prefer to continue extracting the DNA using
their existing protocols. Oragene•DNA is fully compatible
with other manual extraction protocols and automated robots
for high throughput customers.
The documents in this section
will provide you with additional information about purifiying
DNA from Oragene•DNA/saliva samples.
Compatibility
of the AUTOPURE® with Oragene•DNA DNA Collection (pdf): DNA samples collected with Oragene•DNA
and purified with the Autopure LS instrument have an
average DNA yield of 82.3ug and an A260/A280 ratio of
1.87. The DNA is high-molecular weight and suitable
for downstream applications like PCR.
High-throughput
DNA purification with the Qiagen BioRobot EZ1 (pdf):
The BioRobot EZ1 is an automated high-throughput DNA
purification system that uses silica-magnetic-particle
technology. The EZ1 can purify up to six Oragene•DNA/saliva
samples in less than 20 minutes, with excellent DNA
recovery.
High-throughput DNA purification with the Magtration®
12GC (pdf):
The Magtration 12GC is an automated high-throughput
DNA purification system that uses paramagnetic-particle
technology. The 12GC can purify up to twelve Oragene•DNA/saliva
samples in 30 minutes, with excellent DNA yields.
Automated
DNA Purification from Saliva with the AutoGenFlex (pdf):
The AutoGenFlex is a high-throughput instrument that
can extract high molecular weight genomic DNA from
saliva samples collected with Oragene•DNA™. From a
0.5 ml
sample aliquot, the average DNA yield was 15.3 µg
and
the A260/280 ratio was 1.87.
"I am very impressed with the performance of the Oragene•DNA DNA collection technology. We obtained near perfect DNA human identification profile results under very demanding field conditions and as a bonus, reduced our usual extraction time by a factor of 8 and increased total DNA yields by a factor of 30. I wouldn't hesitate to recommend it enthusiastically"
