DNA purification starts
as soon as a donor closes the collection vial and the
saliva is mixed with the Oragene•DNA solution. While the purifier
is included with the Oragene•DNA kits, some researchers and
clinicians prefer to continue extracting the DNA using
their existing protocols. Oragene•DNA is fully compatible
with other manual extraction protocols and automated robots
for high throughput customers.
The documents in this section
will provide you with additional information about purifiying
DNA from Oragene•DNA/saliva samples.
Compatibility
of the AUTOPURE® with Oragene•DNA DNA Collection (pdf): DNA samples collected with Oragene•DNA
and purified with the Autopure LS instrument have an average
DNA yield of 82.3ug and an A260/A280 ratio of 1.87. The
DNA is high-molecular weight and suitable for downstream
applications like PCR.
High-throughput
DNA purification with the Qiagen BioRobot EZ1 (pdf):
The BioRobot EZ1 is an automated high-throughput DNA purification
system that uses silica-magnetic-particle technology.
The EZ1 can purify up to six Oragene•DNA/saliva samples in
less than 20 minutes, with excellent DNA recovery.
High-throughput DNA purification with the Magtration®
12GC (pdf):
The Magtration 12GC is an automated high-throughput DNA
purification system that uses paramagnetic-particle technology.
The 12GC can purify up to twelve Oragene•DNA/saliva samples
in 30 minutes, with excellent DNA yields.
Automated
DNA Purification from Saliva with the AutoGenFlex (pdf):
The AutoGenFlex is a high-throughput instrument that
can extract high molecular weight genomic DNA from
saliva samples collected with Oragene•DNA™. From a 0.5
ml
sample aliquot, the average DNA yield was 15.3 µg
and
the A260/280 ratio was 1.87.
The collection protocol is very convenient, the extraction is very quick and simple, and the yield is great. Our downstream applications include PCR, restriction enzyme digestion and sequencing.
We have collected numerous family members who were not willing to go for a blood draw, as well as children, elderly patients and overseas relatives. The ease of shipping and self-collection are remarkable. Our lab is very impressed with this timely product.
Ruth McPherson, MD, PhD
- Nihan Kavaslar, PhD
Lipoproteins and Atherosclerosis Research Group
University of Ottawa Heart Institute
