DNA from Saliva
Traditional DNA collection methods
such as blood extraction are painful for the donor and
can limit the number of donors that are willing to participate
in an epidemiological study or a clinical trial. As clinicians
look for methods to grow their business or improve patient
care, or as investigators look for ways to increase donor
compliance, it is imperative that non-invasive specimen
collection be a feasible and effective alternative.
The most effective, non-invasive source
of DNA is saliva. DNA in saliva
comes from buccal epithelial cells and white blood cells
found in the mouth.
The Oragene•DNA DNA Self-Collection Kit
is designed to enable researchers and clinicians to
collect reliable saliva samples. Unlike other oral collection
methods, Oragene•DNA yields high-quality, high-quantity
DNA from a small saliva sample. Oragene•DNA is optimized
to preserve and stabilize saliva samples for long term
storage at room temperature without DNA degradation.
DNA from Oragene•DNA is equivalent to DNA from blood.
DNA Genotek has collaborated with vendors
of downstream technologies that rely on high-quality
DNA for analysis. Learn more about how DNA from Oragene•DNA/saliva
samples compares to DNA from blood for sensitive downstream
applications:
SNP
Genotyping of Saliva and DNA using Affymetrix® GeneChip®
Targeted Genotyping System (pdf)
Saliva DNA that is extracted with Oragene•DNA performs equivalent
to or better than blood extracted DNA when used in the
Affymetrix Targeted Genotyping assay.
Comprehensive
Gene Sequence Analysis from Bloodspot and Saliva DNA
(pdf)
DNA yield from 250 µl of saliva is similar to
yield from 1 ml of whole blood, and resulted in similar
performance with the Ambry Genetics TTGE and sequencing
protocols.
The following is a document that discusses
alternate forms of DNA Collection.
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| Title: |
Flow cytometry as
a new method to quantify the cellular content of
human saliva and its relation to gingivitis |
| Authors: |
Johan K.M. Aps,
Karijn Van den Maagdenberg, Joris R. Delanghe,
Luc C. Martens |
| Publication: |
Clinica Chimica Acta
321 (2002) 35-41 |
| Abstract: |
Determining the cellular
content of saliva by means of conventional microscopy
chamber counting is a very
time-consuming and operator-sensitive procedure.
This study concentrated on the use of flow cytometry
to examine the cellular
content of saliva. Erythrocytes, leukocytes, epithelial
cells and bacteria were quantified and the results
were compared with
caries experience and the presence of gingivitis.
Methods: 258 uncentrifuged vortexed paraffin-stimulated
saliva samples (112
males and 146 females) were analyzed with the UF-100R
flow cytometer. Salivary reference values were established
for
erythrocyte, leukocyte, epithelial cell and bacterial
count. Caries experience (DMF) and the presence
of gingivitis were
recorded. Results: Caries experience or caries risk
could not be assessed with flow cytometry. However,
salivary flow cytometry
may be useful in determining an individual’s
risk for gingivitis: a significant increase in salivary
leukocytes was observed in
individuals with gingivitis. At a cut-off level
of 103 leukocytes Al 1 saliva, a sensitivity of
76% and a specificity of 45% was
obtained. Other analytes were not significantly
different between individuals with and without gingivitis.
Conclusion: Flow
cytometry of paraffin-stimulated human saliva seems
a promising diagnostic or predictive tool and further
investigations of
diseases of the oro-pharyngeal loge, such as tonsillitis
and periodontitis, should be carried out in the
future. |
| Link: |
Link
to the publication |
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| Title: |
Criteria for assessing
chronic GVHD |
| Authors: |
H T Greinix, B
Volc-Platzer and R Knobler |
| Publication: |
Bone Marrow Transplantation,
March 2000, Volume 25, Number 5, Pages 575-575 |
| Link: |
Link
to the publication |
| |
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| Title: |
DNA Banking for Epidemiologic
Studies: a Review of Current Practices |
| Authors: |
Steinberg K, Beck
J, Nickerson D, Garcia-Closas M, Gallagher M,
Caggana M, Reid Y, Cosentino M, Ji J, Johnson
D, Hayes R, Earley M, Lorey F, Hannon H, Khoury
M, Sampson E |
| Publication: |
Human Molecular Genetics,
2006 Vol.15, No.3 |
| Abstract: |
To study genetic risk
factors for common diseases, researchers have begun
collecting DNA specimens in large epidemiologic
studies and surveys. However, little information
is available to guide researchers in selecting the
most appropriate specimens. In an effort to gather
the best information for the selection of specimens
for these studies, we convened a meeting of scientists
engaged in DNA-banking for large epidemiologic studies.
In this discussion, we review the information presented
at that meeting in the context of recent published
information. Factors to be considered in choosing
the appropriate specimens for epidemiologic studies
include quality and quantity of DNA, convenience
of collection and storage, cost, and the ability
to accommodate future needs for genotyping. We focus
on four types of specimens that are stored in these
banks: 1) whole blood preserved as dried blood spots,
2) whole blood from which genomic DNA is isolated,
3) immortalized lymphocytes from whole blood or
separated lymphocytes, prepared immediately or subsequent
to cryopreservation, and 4) buccal epithelial cells.
Each of the specimens discussed is useful for epidemiologic
studies according to specific needs which we enumerate
in our conclusions. |
| Link: |
Link
to the publication |
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