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  • How to use OMNIgene•SPUTUM

OMNIgene•SPUTUM (OM-SPD)

Maintain Mycobacterium tuberculosis (MTb) viability while liquefying and decontaminating sputum samples during transport

Overview

OMNIgene®●SPUTUM is a non-toxic and highly stable reagent that liquefies and decontaminates sputum samples at the point-of-collection or in the lab while preserving MTb viability for at least 8 days at ambient temperatures. Optimized samples are compatible with all routine TB tests, enable cost-effective sample transport and simplify laboratory workflows while eliminating the need for NaOH/NALC.

Key Features

  • Stabilize, liquefy and decontaminate samples at point-of-collection
  • Optimize samples for all diagnostic algorithms
  • Eliminate NaOH/NALC to simplify workflows
  • Minimize contamination and cross-contamination

Benefits

  • Enable sample standardization and improve sample quality
  • MTb bacteria remains viable for at least 8 days at ambient temperature
  • Eliminate cost and complexity of cold chain shipping and storage
  • Simplify sputum processing and batching in the lab
  • Eliminate samples lost due to putrefaction
  • Access and transport more samples at less cost

OMNIgene•SPUTUM is a general purpose reagent for in vitro diagnostic use.
Full terms and conditions for all DNA Genotek products are available here.

OMNIgene•SPUTUM (OM-SPD)

Data Sheets

PB-BR-00195: OMNIgene•SPUTUM (OM-SPD) Data Sheet for ROW (pdf)

Videos

MK-00621: Nurse adds OMNIgene•SPUTUM to sputum sample

MK-00630: Patient adds OMNIgene•SPUTUM to sputum sample

MK-00631: OMNIgene•SPUTUM as a replacement for NaOH/NALC

MK-00653: OMNIgene•SPUTUM – Lab processing protocol

Protocols

PD-PR-00596: Protocol for collecting sputum in OMNigene•SPUTUM reagent (pdf)

White Papers

PD-WP-00043: Optimizing high quality samples for Mycobacterium tuberculosis culture with OMNIgene®•SPUTUM (pdf)

PD-WP-00044: Maximizing centralized testing models and GeneXpert® hubs with OMNIgene®•SPUTUM (pdf)

PD-WP-00051: Compatibility of OMNIgene®•SPUTUM and prepIT®•MAX with molecular assays for tuberculosis: Real-time PCR and Hain Lifescience GenoType MTBC line probe assay (pdf)

Material Safety Data Sheets

PD-MSDS-00069: OMNIgene SPUTUM OM-SPD-xxx; OMR-SPD-xxx (pdf)
Téléchargez la version française

Scientific Posters and Publications

Evaluation of a new reagent for preservation of sputum samples for diagnosis Mycobacterium tuberculosis (pdf)

A novel sputum transport solution eliminates cold chain and supports routine tuberculosis testing in Nepal

Evaluation of Mycobacterium tuberculosis viability in OMNIgene•SPUTUM reagent upon multi-day transport at ambient temperature

Evaluation of OMNIgene•SPUTUM for tuberculosis sample processing and liquid culture at a hospital laboratory in India
To request a hardcopy email us at: infectious.disease@dnagenotek.com

Investigation of OMNIgene•SPUTUM performance in delayed tuberculosis testing by smear, culture, and Xpert MTB/RIF assay in Uganda

Evaluation of OMNIgene•SPUTUM reagent for the transport of clinical specimens to reference laboratory without cold chain (pdf)

OMNIgene•SPUTUM (OM-SPD)

What tests are compatible with OMNIgene•SPUTUM?

OMNIgene•SPUTUM is fully compatible with any diagnostic assay, including:

1. Cepheid GeneXpert

OMNIgene•SPUTUM can be used directly with either Xpert® MTB/RIF assay protocol:

OMNIgene•SPUTUM is compatible with the Cepheid® SR buffer and does not need to be removed prior to testing if a centrifuge is not available (see Use Case 3 - Reflex testing: how to run multiple tests from one sample).

2. Liquid Culture

OMNIgene•SPUTUM-treated samples are compatible with liquid culture, including Becton Dickinson BBL® MGIT®.

Samples must be centrifuged according to our recommended protocol in order to remove the reagent prior to inoculating the culture tubes. The reagent is bacteriostatic and will inhibit and/or slow growth in the MGIT tubes if it is inoculated directly.

3. Solid Culture

OMNIgene•SPUTUM-treated samples are compatible with solid culture, including Lowenstein-Jensen medium and Ogawa-Kudoh method.

Samples must be centrifuged according to our recommended protocol in order to remove the reagent prior to inoculating the culture tubes. The reagent is bacteriostatic and will inhibit and/or slow growth if it is inoculated directly onto the agar.

Using the Ogawa-Kudoh method with OMNIgene•SPUTUM

  1. Add OMNIgene•SPUTUM to the sputum sample at approximately 1:1 volume.
  2. Secure the cap of the specimen cup or tube tightly; mix the contents by inverting 10x.
  3. Swirl a sterile cotton swab in the mixture.
  4. Streak the swab on the Ogawa-Kudoh media slant.
  5. Incubate at 37°C.

4. Molecular Assays

OMNIgene•SPUTUM-treated samples are compatible with molecular assays, including line probe assays, PCR, targeted and whole genome sequencing.

Follow our recommended protocol to prepare sediments and extract DNA using your preferred extraction method. We suggest using prepIT®•MAX to prepare DNA.

What other sample types can I use with OMNIgene•SPUTUM?

1. Extrapulmonary Tuberculosis (EPTB)

Contact us for more information.

2. Feces

Contact us for protocols.

3. Urine

Contact us for more information.

Can I use OMNIgene•SPUTUM for Non-Tuberculosis Mycobacteria (NTMs)?

Contact us for more information.

OMNIgene•SPUTUM is a general purpose reagent for in vitro diagnostic use.
Full terms and conditions for all DNA Genotek products are available here.


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