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"I found the Oragene/saliva sample collection kit very simple to use. The protocol was easy to follow and time-saving permitting me to perform the extraction amplification and analysis steps in a single day."

Melissa Boweman,
Technologist
Plexagen Diagnostics Ltd.

Select Your Product:
  • Overview
  • Literature

Oragene•ONE (ON-500)
0.5 mL sample volume

Collect superior samples for your genetic analysis and testing

All genetic analysis starts with the collection of DNA samples. Collect reliable samples with an all-in-one system for the collection, stabilization and transportation of DNA from saliva.

  • Improve donor care and compliance with painless, non-invasive sample collection
  • Eliminate phlebotomy costs
  • Ideal for use with children or patients that will not comply with blood collections
  • Increase efficiency, minimize sample handling and reduce handling errors with a compatible format for high-throughput processing
  • Sample remains stable at room temperature, reducing transportation and storage costs
  • Sample can be mailed using the standard postal system
  • DNA from saliva is equivalent to DNA from blood for downstream applications

Collection method comparison

Blood Collection
Oral Collection
Attributes
Venous blood
Mouthwash
Buccal swabs
Oragene•ONE
(ON-500)
Non-invasive collection
Standardized format for high-throughput processing
Specimen stability at room temperature
Days
Weeks
Days
Months
Low bacterial content
✘††
(up to 60%
bacterial content)
✘††
(up to 90%
bacterial content)
✔†
(median 7.4%
bacterial content)
Median DNA yield
30 µg
35 µg
2 µg
12 µg†
Sample size
1 mL
10 mL‡
1 swab
0.5 mL
Molecular weight
> 23 kb
> 23 kb
< 23 kb
> 23 kb
Shipping at ambient temperature
Full customization available

† Iwasiow, R.M., Tayeb, M.A. and James, C.M.P. (2009). Quality, yield and bacterial content of DNA from human saliva collected and purified using Oragene•ONE. DNA Genotek. PD-WP-013.
†† Birnboim, H.C., Iwasiow, R.M. and James, C.M.P. (2008). Human genomic DNA content of saliva samples collected with the Oragene self-collection kit. DNA Genotek. PD-WP-011.
‡ Volume of mouthwash used for sample collection.

Full terms and conditions for all DNA Genotek products are available here.

Oragene•ONE (ON-500)
0.5 mL sample volume

Data Sheets

Other Languages:

MK-BR-018: Event-based Collection (pdf)

MK-BR-00026: Service and support – DNA Genotek's commitment (pdf)

MK-00493: Infographic – The Oragene Advantage – DNA from saliva is driving genetic discoveries.

MK-00098: Infographic – DNA from saliva vs blood. Who wins the cost battle?

Video

What discoveries will you make using Oragene?

What would you tell a peer about Oragene?

Impact of sample type and analytical methods on NGS data quality.

Calvin Harley, Ph.D., of Telomere Diagnostics shares his clinical research work into the impact of telomere length on health and aging at the 2013 European Society for Human Genetics conference.

Why I use Oragene. Watch this video and learn why so many customers choose Oragene.

Protocols

Laboratory protocols for manual purification of DNA using DNA Genotek's prepIT•L2P DNA extraction kit

White Papers

PD-WP-009: Shipping recommendations for exempt specimen samples (pdf)

PD-WP-013: Quality, yield and bacterial content of DNA from human saliva collected and purified using Oragene®•ONE (pdf)

User Instructions

Complete ON-500 Collection Instructions

Material Safety Data Sheet

PD-MSDS-00005: Oragene•ONE (ON-500) (pdf)
Téléchargez la version française

Accessories

Mailing Accessories

Storage Accessories

Customization


DNA Genotek's sample collection devices and nucleic acid stabilization chemistries are protected by issued and pending patents in numerous countries around the world.
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