Humans share 7% of their DNA with E. coli bacterium.
Using Saliva Sponges to Collect DNA Samples from Infants & Young Children – CS1 with the OG-250
Place the saliva sponge into the child's mouth in the cheek pouch (the space between the gums and the inner cheek). Gently move the saliva sponge around the upper and lower cheek pouches on both sides of the mouth to soak up as much saliva as possible. There is no need to 'scrape' the inner cheek with saliva sponges – simply collect as much saliva as possible from the cheek pouches. The sponge will absorb more saliva if it is left in the child's mouth for a longer time (up to 60 seconds).
Once collected, cut the sponge into the blue base of the Oragene•DNA kit as follows. Place the sponge firmly against the bottom of the kit between the tooth and the kit wall (see picture above). This action will ensure that the sponge tip remains in the container during the cutting action. Using the scissors provided, cut the narrow part of the handle just above the sponge.
Carefully cap the kit and tighten it firmly. Once the Oragene•DNA liquid is released from the cap, it with preserve the DNA collected by the sponge(s).
Invert gently 5 times to mix the sample. If the scissors are to be re-used, they should be rinsed with tap water and wiped dry between donors.
"I found the Oragene•DNA collection kit very simple to use. The protocol was easy to follow and time-saving permitting me to perform the extraction amplification and analysis steps in a single day."
