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Sample Collection | Storage | Purification | Downstream Applications

Sample Collection

Genetic research starts with the collection of DNA samples. Oragene•DNA is the easiest way to collect large amounts of DNA from more donors, with the least cost and complexity. Oragene•DNA is ideal for mailing studies as the compact, robust design fits through mailbox slots and securely protects the sample.

Easy and Non-Invasive Collection
Donor delivers saliva
into the Oragene•DNA
collection container.
Capping the container releases
DNA-preserving fluid which then mixes
with the saliva. DNA is now stabilized
at room temperature until it is analyzed
at the lab.

The documents on this page will provide you with additional information about DNA collection using Oragene•DNA.

DNA Collection with Saliva

DNA Yield with Oragene•DNA (pdf):
Oragene•DNA gives higher yields than other oral collection methods. The median yield from Oragene•DNA samples is 110 µg. In comparison, buccal swabs may yield as little as 1.9 µg of DNA.

Mailing Solutions (pdf):
For researchers who are collecting from remote donors, DNA Genotek has developed a full mailing solution. This solution includes a summary of the International Air Transport Association guidelines for shipping biological samples.

Maximizing DNA yield with Oragene•DNA (pdf):
Téléchargez la version française
Tips for improving the median amount of DNA per sample.

DNA Yield is Proportional to Saliva Volume (pdf):
The DNA yield from Oragene•DNA is proportional to the amount of saliva. Less than 2 ml of saliva results in proportionately less DNA yield.

 

"The collection protocol is very convenient, the extraction is very quick and simple, and the yield is great. Our downstream applications include PCR, restriction enzyme digestion and sequencing.

We have collected numerous family members who were not willing to go for a blood draw, as well as children, elderly patients and overseas relatives. The ease of shipping and self-collection are remarkable. Our lab is very impressed with this timely product."

Ruth McPherson, MD, PhD and Nihan Kavaslar, PhD
Lipoproteins and Atherosclerosis Research Group, University of Ottawa Heart Institute

© 2010 DNA Genotek Inc.